The absorbance was measured using MULTISKAN JX (Thermo Fisher Scientific, ... Ascent Software for Multiskan Ascent (Thermo Fisher Scientific, Worsham, MA, USA). ... Funding: KY (17K10241) received grant for scientific research from the .... ... an ELISA reader (Multiskan Ascent, Labsystem, Vantaa, Finland) at 450 nm. 2.8. Th1-Th17 Phenotipagem Spleens were aseptically removed and splenocytes were ... permeabilized using eBiosciences' intracellular fixation (Thermo Scientific, ... (BD Biosciences) and analyzed with the flow cytometer's proprietary software.
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